槐定碱致癫痫样作用研究
投稿时间:2009-08-20    责任编辑:  点此下载全文
引用本文:彭晓东,陆钊罡,牟青春,侯延辉.槐定碱致癫痫样作用研究[J].中国中药杂志,2010,35(1):122.
DOI:10.4268/cjcmm20100126
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作者中文名作者英文名单位中文名单位英文名E-Mail
彭晓东 PENG Xiaodong 宁夏医科大学 基础医学院 药理学教研室,宁夏 银川 750004 Department of Pharmacology, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China  
陆钊罡 LU Zhaogang 宁夏医科大学 基础医学院 药理学教研室,宁夏 银川 750004 Department of Pharmacology, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China  
牟青春 MU Qingchun 宁夏医科大学 基础医学院 药理学教研室,宁夏 银川 750004 Department of Pharmacology, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China  
侯延辉 HOU Yanhui 宁夏医科大学 基础医学院 药理学教研室,宁夏 银川 750004 Department of Pharmacology, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China  
基金项目:宁夏2007自然科学基金项目(NZ0785);宁夏自治区教育厅2006年度高等学校科学研究项目
中文摘要:目的: 观察槐定碱(sophoridine)致痫大鼠清醒核团脑电(IEEG)变化特点;研究痫样特征分类和致痫作用机制。 方法 :采用慢性埋藏电极记录清醒大鼠核团脑电(intracranial electroencepholography, IEEG)的方法,观察槐定碱对大鼠产生痫样放电的特点,并判断致痫原发部位;采用小鼠模型实施工具药拮抗实验,探讨槐定碱所致癫痫样发作的作用机制及类型。 结果 :海马齿状回(DG)内颗粒细胞对于槐定碱致痫作用最敏感,其次是内侧穿行通路(PP)和颞叶皮层(TC)。阈下催眠剂量的地西泮、催眠剂量的巴比妥钠可以对抗槐定碱引起的痫样惊厥的发生;对抗最大电休克剂量的苯妥英钠不能对抗槐定碱引起的痫样惊厥的发生,但可以减缓惊厥的发生时间和小鼠的死亡时间。 结论 :海马内部异常放电在槐定碱致痫作用中可能起到重要作用,海马部位可能是痫样发作的原发部位;槐定碱的致动物癫痫样发作属于临床小发作类型,地西泮是较理想的预防药物。
中文关键词:槐定碱  脑电波  癫痫  地西泮  戊巴比妥钠  苯妥英钠
 
Epileptic seizure-like effect of Sophora alkaloid sophoridineon experimental animals
Abstract:Objective : To investigate the epileptic seizure-like effect of Sophora alkaloid sophoridine on electroencepholography (EEG) and its possible characteristic and the mechanism of the seizure-like effect. Method : Chronic electron implantation was employed for the intracranial electroencepholography (IEEG) recording in rat, and the traditional anti-seizure drugs were for the mechanism study in mice. Result : Compared with the medial perforant path (PP) area and the temporal cortex (TC) , the granule cells in hippocampus dentate gyrus (DG)area is more sensitive in the kindling effect by sc sophoridine. Under-threshold hypnotic dosage of diazepam and the hypnotic dosage of pentobarbital sodium can block the sophoridine kinded seizure in mice, but the phenytoin sodium can not block the seizure, also the dosage of it can block the maximal electroconvulsive shock (MES) seizure. Conclusion : Sophoridine-induced synchronous oscillations in the hippocampus could elicit the generation and development of seizure. And the hippocampus might play the crucial role and be the original part of the seizure.Sophoridine kinded seizure might belong to clonic seizures, and the diazepam is the ideal agent for the treatment.
keywords:sophoridine  EEG  seizure  diazepam  pentobarbital sodium  phenytoin sodium
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