吴茱萸水提物给药大鼠尿液代谢组学研究
投稿时间:2009-01-20    责任编辑:  点此下载全文
引用本文:张启云,徐国良,马晓雪,曾治君,黄丽萍,余日跃,刘红宁.吴茱萸水提物给药大鼠尿液代谢组学研究[J].中国中药杂志,2010,35(1):99.
DOI:10.4268/cjcmm20100121
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作者中文名作者英文名单位中文名单位英文名E-Mail
张启云 ZHANG Qiyun 江西中医学院 现代中药制剂教育部重点实验室, 江西 南昌 330006 Key Laboratory of Modern Preparation, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China  
徐国良 XU Guoliang 江西中医学院 现代中药制剂教育部重点实验室, 江西 南昌 330006 Key Laboratory of Modern Preparation, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China  
马晓雪 MA Xiaoxue 江西中医学院 现代中药制剂教育部重点实验室, 江西 南昌 330006 Key Laboratory of Modern Preparation, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China  
曾治君 ZENG Zhijun 江西中医学院 现代中药制剂教育部重点实验室, 江西 南昌 330006 Key Laboratory of Modern Preparation, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China  
黄丽萍 HUANG Liping 江西中医学院, 江西 南昌 330006 Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China  
余日跃 YU Riyue 江西中医学院, 江西 南昌 330006 Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China  
刘红宁 LIU Hongning 江西中医学院 现代中药制剂教育部重点实验室, 江西 南昌 330006 Key Laboratory of Modern Preparation, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China lhongning@yahoo.com.cn 
基金项目:国家重点基础研究发展计划(973)项目(2006CB504702)
中文摘要:目的: 研究吴茱萸水提液给药对大鼠内源性代谢产物的影响。 方法: 吴茱萸水提物按0.385 7 g·kg-1给SD大鼠连续灌胃33 d,收集尿液,通过Agilent 1200 6410三重四极杆质谱对尿液中内源性代谢物进行全扫描分析,SIMCA-P软件经过PCA,PLS-DA分析。 结果: 给药组与对照组相比,大鼠尿液代谢物白细胞三烯,棕榈酰乙醇胺,二十二碳六烯酸,8(R)-氢过氧亚油酸/花生酸等有明显变化。 结论: 吴茱萸水提物灌胃对正常大鼠机体代谢产生影响,为进一步阐释吴茱萸的药性研究工作提供依据。
中文关键词:液质联用  代谢组学  吴茱萸  生物标记物
 
Preliminary study of metabonomcs on aqueous extract ofEvodia rutaecarpa in sprague-dawley rats
Abstract:Objective: To study the change of endogenous metabolites of SD rats administrated of aqueous extract of Evodiae rutaecarpa. Method: Six SD rats had been successively administrated aqueous extract of E. rutaecarpa(0.385 7 g·kg-1) for 33 days. An agilent 1200 6410 triplequadrupole mass spectrometer was used for the analysis of endogenous metabolites in rat urine samples. These data was analyzed by the principal component analysis (PCA) and PLS-DA using the SIMCA-P 10.0 software. Result: The significant difference in metabolic profiles between the control group and the dosed group was well observed by PCA of the MS data. Conclusion: The E. rulaecarpa has changed the endogenous metabolites of SD rats. This work can provide the base for the further research on the interpretation of drug property of E. rulaecarpa.
keywords:LC-MS/MS  metabonomics  Evodia rutaecarpa  biomarker
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