白藜芦醇对缺氧诱导大鼠乳鼠心肌细胞损伤的保护作用
投稿时间:2009-06-10    责任编辑:  点此下载全文
引用本文:黄秀兰,周秋兰,古丽丽,刘丹妮,李志勇,刘庆山,朱丹.白藜芦醇对缺氧诱导大鼠乳鼠心肌细胞损伤的保护作用[J].中国中药杂志,2010,35(1):94.
DOI:10.4268/cjcmm20100120
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作者中文名作者英文名单位中文名单位英文名E-Mail
黄秀兰 HUANG Xiulan 中央民族大学 中国少数民族传统医学研究院, 北京 100081 Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China hxlcun@sina.com 
周秋兰 ZHOU Qiulan 中央民族大学 中国少数民族传统医学研究院, 北京 100081 Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China  
古丽丽 GU Lili 中央民族大学 中国少数民族传统医学研究院, 北京 100081 Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China  
刘丹妮 LIU Danni 中央民族大学 中国少数民族传统医学研究院, 北京 100081 Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China  
李志勇 LI Zhiyong 中央民族大学 中国少数民族传统医学研究院, 北京 100081 Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China  
刘庆山 LIU Qingshan 中央民族大学 中国少数民族传统医学研究院, 北京 100081 Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China  
朱丹 ZHU Dan 中央民族大学 中国少数民族传统医学研究院, 北京 100081 Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China  
基金项目:国家民族事务委员会项目(08 zy 13);中央民族大学"985工程"项目
中文摘要:目的: 观察白藜芦醇(Res)对缺氧诱导大鼠乳鼠心肌细胞损伤的保护作用。 方法 : 建立体外培养大鼠乳鼠心肌细胞缺氧模型, MTT法检测心肌细胞活力,免疫组织化学法检测心肌细胞缺氧诱导因子-1α(HIF-1α)表达水平,检测心肌细胞培养液中乳酸脱氢酶(LDH)水平和心肌细胞内谷胱甘肽过氧化物酶(GSH-Px)活性。 结果 : 大鼠乳鼠心肌细胞在体外缺氧培养24 h后,HIF-1α表达水平明显升高;心肌细胞培养液中LDH活性从正常对照组的(93.07 ±15.84) U·L-1上升到(750.77±181.51)U·L-1(P<0.01);心肌细胞内GSH-Px活性从正常对照组(46.96±8.36) U·mL-1下降为(27.13±4.76) U·mL-1(P<0.01)。25,50,75 μmol·L-1 Res可剂量依赖性抑制缺氧诱导大鼠乳鼠心肌细胞内HIF-1α表达增加;心肌细胞培养液中LDH含量呈剂量依赖性下降,分别为(486.17±69.97),(189.43±32.07),(155.34±29.57) U·L-1(P<0.05或P<0.01);心肌细胞内GSH-Px活性呈剂量依赖性升高,分别为(33.55±6.34),(37.67±6.73),(41.44±7.91) U·mL-1(P<0.05或P<0.01)。 结论 : Res对缺氧诱导大鼠乳鼠心肌细胞损伤有良好保护作用。
中文关键词:白藜芦醇  大鼠乳鼠心肌细胞  缺氧  缺氧诱导因子-1α
 
Protective effects of resveratrol on neonatal rat cardiomyocytelesion induced by hypoxia
Abstract:Objective : To investigate the effects of resveratrol(Res)on neonatal rat cardiomyocyte lesion induced by hypoxia. Method : The cardiomyocyte of neonatal rats were cultured in vitro and the model of cardiomyocyte hypoxia was established. The cardiomyocyte vitalities were determined by MTT assay, the HIF-1α expression levels in myocardial cells was detected by immunohistochemical , the activities of peroxidase(GSH-Px) and lactate dehydrogenase(LDH) were measured as well. Result : After the administration of hypoxia for 24 hours, the HIF-1α expression in myocardial cells was significantly increased. The LDH level in the culture medium was increased from (93.07±15.84) U·L-1 to ( 750.77±181.51) U·L-1 (P<0.01). The intracellular GSH-Px activity was decreased from (46.96±8.36) U·mL-1 to (27.13±4.76) U·mL-1 (P<0.05). Res 25,50 and 75 μmol·L-1 could dose-dependently inhibit the raising of the HIF-1α expression in myocardial cells induced by hypoxia. The LDH activities were decreased dose- dependently to (486.17±69.97),(189.43±32.07), (155.34 ±29.57) U·L-1, respectively (P<0.05 or P<0.01). The GSH-Px activities were increased dose-dependently (33.55±6.34), (37.67±6.73), ( 41.44±7.91) U·mL-1 (P<0.05 or P<0.01 ). Conclusion : Res has a protective effect on neonatal rat cardiomyocyte lesion induced by hypoxia.
keywords:resveratrol  neonatal rat cardiomyocyte  hypoxia  hypoxia-inducible factor-1 alpha
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