4种延胡索成分对乳鼠心肌细胞缺氧和过氧化损伤的影响
投稿时间:2009-06-12    责任编辑:  点此下载全文
引用本文:李澎,任钧国,段昌令,林成仁,刘建勋.4种延胡索成分对乳鼠心肌细胞缺氧和过氧化损伤的影响[J].中国中药杂志,2010,35(1):84.
DOI:10.4268/cjcmm20100118
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作者中文名作者英文名单位中文名单位英文名E-Mail
李澎 LI Peng 中国中医科学院 西苑医院,北京 100091 Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China  
任钧国 REN Junguo 中国中医科学院 西苑医院,北京 100091 Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China  
段昌令 DUAN Changling 中国中医科学院 西苑医院,北京 100091 Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China  
林成仁 LIN Chengren 中国中医科学院 西苑医院,北京 100091 Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China  
刘建勋 LIU Jianxun 中国中医科学院 西苑医院,北京 100091 Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China liujx0324@sina.com 
基金项目:国家"十一五"科技支撑计划课题(2006BAI08B04-08);国家自然科学基金重点项目(30830118)
中文摘要:目的: 观察延胡索乙素、脱氢紫堇碱、黄连素和巴马汀4种单体对心肌细胞缺氧、过氧化损伤的影响,探讨延胡索抗心肌缺血作用的物质基础。 方法 :体外培养乳鼠心肌细胞,复制缺氧复氧和过氧化氢损伤模型;使用4种单体与细胞共孵育;MTT法测定细胞活力,评价药物的安全作用范围和对过氧化氢损伤的作用;酶反应动力监测法测定LDH活性,计算细胞LDH漏出量和药物对LDH漏出的抑制率,评价药物对缺氧损伤的作用。 结果 :多至500 mg·L-1的延胡索乙素对细胞活力无任何影响,脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3, 0.6,6.3 mg·L-1时,显著降低细胞活力(P<0.05,P<0.01);延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50~100, 1.25~5, 4,30 mg·L-1时,显著抑制缺氧复氧导致的LDH漏出(P<0.05,P<0.01);4种单体对过氧化氢损伤无显著的保护作用。 结论 :延胡索抗心肌缺血损伤的作用与其成分延胡索乙素、脱氢紫堇碱、黄连素和巴马汀对心肌细胞的直接保护能力有关;其中,延胡索乙素和脱氢紫堇碱的作用最为重要,前者安全性高,但效能较低,后者安全性低,而效能较高;而这4个单体的保护作用可能是通过抗氧化损伤之外的机制达到的。
中文关键词:延胡索  成分  心肌细胞  缺氧复氧损伤
 
Effects of four components of Rhizoma Corydalis on anoxia andperoxidation injuries in neonatal cardiomyocytes
Abstract:Objective : To observe the effects of tetrahydropalmatine, dehydrocorydaline, berberine and palmatine on anoxia and peroxidation injuries in cardiomyocytes, and study the marterial basis of the anti-ischemia effect on myocardium of Rhizoma Corydalis. Method : Neonatal rat cardiomyocytes were cultured in vitro, and subjected to an anoxia-reoxia and the hydrogen peroxide injury models. The four compounds were added into the culture medium. The cell viability was measured by MTT method to determine the safe concentrations and the anti- hydrogen peroxide injury effects of the compounds. The LDH activity in culture mediums was measured with the enzyme reaction dynamics-monitoring method to value the anti-anoxia injury effects of the compounds. Result : At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability, while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively(P<0.05 or P<0.01). Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively(P<0.05 or P<0.01). None of the four compounds showed significant effect on the hydrogen peroxide injury. Conclusion : The anti-ischemia effect in myocardium of Rhizoma Corydalis is related to the direct protective effects on cardiomyocytes of its components, tetrahydropalmatine, dehydrocorydaline, berberine and palmatine, amomg which tetrahydropalmatine and dehydrocorydaline are the most important, the former with high safety and low efficacy, while the latter with low safety and high efficacy. And the direct protective effects on cardiomyocytes of these four components may be attained through mechanisms other than anti-peroxidation.
keywords:Rhizoma Corydalis  components  cardiomyocyte  anoxia-reoxia
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