IgG启动子探针对中药注射剂进行临床前安全性评价的研究
投稿时间:2009-09-02    责任编辑:  点此下载全文
引用本文:王玉刚,李乐乐,雷帆,梁爱华,邢东明,杜力军.IgG启动子探针对中药注射剂进行临床前安全性评价的研究[J].中国中药杂志,2010,35(1):76.
DOI:10.4268/cjcmm20100116
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作者中文名作者英文名单位中文名单位英文名E-Mail
王玉刚 WANG Yugang 清华大学 生物科学与技术系, 医学院 药学系 药物药理研究室, 北京 100084 Laboratory of Pharmaceutical Science, Department of Biological Sciences and Biotechnology, Department ofPharmacology and Pharmaceutical Science, School of Medicine, Tsignghua University, Beijing 100084, China  
李乐乐 LI Lele 清华大学 生物科学与技术系, 医学院 药学系 药物药理研究室, 北京 100084 Laboratory of Pharmaceutical Science, Department of Biological Sciences and Biotechnology, Department ofPharmacology and Pharmaceutical Science, School of Medicine, Tsignghua University, Beijing 100084, China  
雷帆 LEI Fan 清华大学 生物科学与技术系, 医学院 药学系 药物药理研究室, 北京 100084 Laboratory of Pharmaceutical Science, Department of Biological Sciences and Biotechnology, Department ofPharmacology and Pharmaceutical Science, School of Medicine, Tsignghua University, Beijing 100084, China  
梁爱华 LIANG Aihua 中国中医科学院 中药研究所, 北京 100700 Institute of Chinese Material Medica, Chinese Academy of Traditional Chinese Medicine, Beijing 100700, China  
邢东明 XING Dongming 清华大学 生物科学与技术系, 医学院 药学系 药物药理研究室, 北京 100084 Laboratory of Pharmaceutical Science, Department of Biological Sciences and Biotechnology, Department ofPharmacology and Pharmaceutical Science, School of Medicine, Tsignghua University, Beijing 100084, China  
杜力军 DU Lijun 清华大学 生物科学与技术系, 医学院 药学系 药物药理研究室, 北京 100084 Laboratory of Pharmaceutical Science, Department of Biological Sciences and Biotechnology, Department ofPharmacology and Pharmaceutical Science, School of Medicine, Tsignghua University, Beijing 100084, China lijundu@mail.tsinghua.edu.cn 
基金项目:国家自然科学基金项目(30801523);国家"十一五"科技支撑计划(2006BAI08B03-09);中国博士后科学基金(20080440418);"重大新药创制"科技重大专项(2009ZX09103-301, 2009ZX09502-021)
中文摘要:目的: 利用分子生物技术构建免疫球蛋白G(IgG)启动子调控绿色荧光蛋白(green fluorescent protein, GFP)转基因细胞,尝试以IgG基因表达起始为标准在临床前对中药注射剂进行其诱导II型变态反应的可能性预测。 方法 :构建IgG启动子调控绿色荧光蛋白表达的转基因细胞系,用葛根素,KCl,聚氧乙烯脱水山梨醇单油酸酯(吐温80)对该系统进行测试,并尝试用该系统对不同批次鱼腥草注射液进行筛选评价,观察统计各给药组中发光细胞数量的变化。 结果 :非离子表面活性剂吐温80对IgG表达启动过程有抑制作用,葛根素能显著激活IgG基因的表达,KCl对IgG表达无作用。所测批次鱼腥草注射液对IgG表达没有激活作用。 结论 :IgG启动子调控绿色荧光蛋白表达细胞模型能在IgG表达层次评价药物致敏性,吐温80能在转录水平上抑制IgG的表达,所测批次鱼腥草注射液在IgG转录层次上无诱导II型变态反应活性。
中文关键词:IgG  II型变态反应  鱼腥草注射液  葛根素  转录调控
 
Study on IgG promoter as probe to evaluate safetyof injections in pre-clinic
Abstract:Objective : To construct an effective system screening and evaluating possible injections and components inducing allergy type II. Method : Transfect IgG promoter-regulated green fluorescent protein expressing plasmid into RPMI-8226 cell. The number of fluorescent cells after drug treatment was calculated and statistically evaluated. Result : Tween 80 can suppress the expression of IgG effectively, and puerarin has activity of stimulating IgG expression, and the system has no response to KCl-treatment. No effect of Yuxingcao(Herba Houttuyniae) injection on this system is observed. Conclusion : IgG promoter-drove green fluorescent protein expressing cell line can be used as system screening injections and components inducing allergy type II based on stimulating IgG promoter activity. Tween 80 can suppress the expression of IgG at the transcriptional level. Four batches of Yuxingcao injection cannot induce allergy type II by activating IgG expression.
keywords:IgG  allergy type II  Herba Houttuyniae  puerarin  transcriptional regulation
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