加压溶剂法提取雷公藤多苷及其条件优化
投稿时间:2009-09-17    责任编辑:  点此下载全文
引用本文:杨磊,李彤,祖元刚.加压溶剂法提取雷公藤多苷及其条件优化[J].中国中药杂志,2010,35(1):44.
DOI:10.4268/cjcmm20100109
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作者中文名作者英文名单位中文名单位英文名E-Mail
杨磊 YANG Lei 东北林业大学 森林植物生态学教育部重点实验室, 黑龙江 哈尔滨 150040 Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040,China  
李彤 LI Tong 东北林业大学 森林植物生态学教育部重点实验室, 黑龙江 哈尔滨 150040 Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040,China  
祖元刚 ZU Yuangang 东北林业大学 森林植物生态学教育部重点实验室, 黑龙江 哈尔滨 150040 Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040,China zygorl@126.com 
基金项目:国家"十一五"科技支撑计划项目(2006BAD18B04)
中文摘要:目的: 对卫矛科药用植物雷公藤提取方法进行研究。 方法 :利用加压溶剂提取方法进行提取分离,根据理化性质用显色-比色法进行定量分析,并在单因素试验基础上,根据中心组合试验设计原理采用3因素3水平的响应面分析法进行工艺优化。 结果 :经过响应面分析法得出优化的工艺条件为料液比1 ∶ 9.5,提取温度115 ℃,提取时间80 min,在此条件下雷公藤多苷的浸膏得率可达0.21%,纯度为0.52%。 结论 :加压溶剂提取方法与常规提取法相比优势明显,是一种有效的提取分离方法。
中文关键词:雷公藤  雷公藤多苷  加压提取  响应面
 
Pressurized liquid extraction of Tripterygiumw wilfordii polyglycosides andoptimization of extraction conditions
Abstract:Objective To investigate the extraction method of polyglycosides from Tripterygium wilfordii. Method : The extraction method of pressurized liquid extraction was employed and chromogenic colorimetric technique were used for quantitative analysis. Based on the single-factor experiment,according to the center combination design this paper used three factors and three levels of response surface methodology for process optimization. Result : The optimized conditions were as follows: ratio of solid to liquid was 1 ∶ 9.5,at the temperature of 115 ℃ for 80 minutes,the actual extract ratio and purity of polyglycosides obtained were 0.21% and 0.52%, respectively. Conclusion : The method of pressurized liquid extraction has obvious advantages over conventional reflux extracting.
keywords:Tripterygium wilfordii  polyglycosides  pressurized liquid extraction  response surface methodology
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