离体培养条件对人参不定根生长及其活性成分合成的影响
投稿时间:2009-04-11    责任编辑:  点此下载全文
引用本文:黄滔,高文远,王娟,曹宇.离体培养条件对人参不定根生长及其活性成分合成的影响[J].中国中药杂志,2010,35(1):13.
DOI:10.4268/cjcmm20100102
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作者中文名作者英文名单位中文名单位英文名E-Mail
黄滔 HUANG Tao 天津大学 药物科学与技术学院, 天津 300072
天津科技大学 中药生物工程研究所, 天津 300457
College of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China
Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Scienceand Technology, Tianjin 300457, China
 
高文远 GAO Wenyuan 天津大学 药物科学与技术学院, 天津 300072
天津科技大学 中药生物工程研究所, 天津 300457
College of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China
Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Scienceand Technology, Tianjin 300457, China
pharmgao@tju.edu.cn 
王娟 WANG Juan 天津大学 药物科学与技术学院, 天津 300072 College of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China  
曹宇 CAO Yu 天津科技大学 中药生物工程研究所, 天津 300457 Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Scienceand Technology, Tianjin 300457, China  
基金项目:国家"十一五"科技支撑计划项目(2006BAI06A16-02)
中文摘要:目的: 对人参不定根的摇瓶培养条件进行系统的优化。 方法 :利用组织培养技术结合高效液相色谱法和紫外分光光度法,考察了接种量、蔗糖浓度以及无机盐浓度对人参不定根的生长、人参皂苷以及人参多糖合成的影响。 结果 :每1 L培养基接种的不定根鲜重为20 g时人参不定根的干重增殖倍数达到最大值;随着蔗糖浓度的升高,人参不定根干重增殖倍数呈先升高后降低的趋势,人参多糖含量增长趋势不明显,不同蔗糖浓度时各单体皂苷含量有显著区别,人参总皂苷含量随蔗糖浓度的升高而降低,单位体积培养基中的多糖和皂苷产量均在40 g·L-1蔗糖质量浓度下达到最大值;培养基中的无机盐浓度对不定根的生长以及多糖和皂苷的合成与积累都有较大影响,3/4MS最有利于不定根的生长以及皂苷的合成,而不定根中的多糖含量则随着盐浓度的升高而降低。 结论 :接种量、蔗糖浓度、无机盐浓度都会显著影响人参不定根的生长以及其活性成分的合成和积累。
中文关键词:人参  不定根  接种量  蔗糖浓度  无机盐浓度  人参皂苷  人参多糖
 
Effects of culture conditions on biomass and active components ofadventitious roots culture in Panax ginseng
Abstract:Objective : To optimize the culture condition of adventitious roots of Panax ginseng. Method : The adventitious roots were obtained through tissue culture by manipulation of inoculum, various sucrose concentrations and salt strength. The contents of ginsenosides Re, Rb1 and Rg1 were determined by HPLC while the contents of polysaccharides were determined by ultraviolet spectrophotometry. Result : The multiplication of adventitious roots reached the peak when the inoculum was 20 g·L-1. The effects of sucrose concentration and salt strength on adventitious roots were observed. The contents of polysaccharides were higher when the medium contained more sucrose. 40 g·L-1 sucrose was favorable for roots growth and biosynthesis of Re, while 30 g·L-1 was favorable for the biosynthesis of Rb1 and Rg1. 3/4MS medium was benefit for the growth of adventitious roots and the biosynthesis of ginsenosides. The contents of polysaccharides were decreased with the increase of salt strength. Conclusion : The results showed that inoculum, various sucrose concentrations and salt strength have significant influences on adventitious roots growth, secondary metabolite and polysaccharide synthesis in P. ginseng.
keywords:Panax ginseng  adventitious roots  inoculum  sucrose concentration  salt strength  ginsenosides  polysaccharides
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