人工栽培铁皮石斛多糖含量变异规律
投稿时间:2009-07-09    责任编辑:  点此下载全文
引用本文:诸燕,斯金平,郭宝林,何伯伟,张爱莲.人工栽培铁皮石斛多糖含量变异规律[J].中国中药杂志,2010,35(4):427.
DOI:10.4268/cjcmm20100406
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作者中文名作者英文名单位中文名单位英文名E-Mail
诸燕 ZHU Yan 浙江林学院 天然药物研发中心,浙江 临安 311300 Centre for Research and Development of Natural Medicines of Zhejiang Forestry University, Lin'an 311300, China  
斯金平 SI Jinping 浙江林学院 天然药物研发中心,浙江 临安 311300 Centre for Research and Development of Natural Medicines of Zhejiang Forestry University, Lin'an 311300, China lssjp@163.com 
郭宝林 GUO Baolin 中国医学科学院 药用植物研究所,北京 100094 China Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking UnionMedical College, Beijing 100094,China  
何伯伟 HE Bowei 浙江省中药材产业协会,浙江 杭州 310020 Zhejiang Industry of Chinese Medical Material Association, Hangzhou 310020,China  
张爱莲 ZHANG Ailian 浙江林学院 天然药物研发中心,浙江 临安 311300 Centre for Research and Development of Natural Medicines of Zhejiang Forestry University, Lin'an 311300, China  
基金项目:浙江省重大科技专项(2007C13029);浙江省重大林业科技项目(2007A12)
中文摘要:目的: 全面了解人工栽培铁皮石斛药材多糖质量分数,以及种质、采收年限等因素与多糖质量分数的关系,为优质铁皮石斛药材培育提供依据。 方法 :采集浙江铁皮石斛人工设施栽培骨干基地(产量占全国90%以上)11个种质一至三年生(生理年龄)的33个样品,并分别记载其形态特征;采用苯酚-硫酸比色法测定铁皮石斛多糖质量分数。 结果 :全国铁皮石斛骨干企业生产的二年生铁皮石斛其多糖平均质量分数达34.47%(25.63%~41.65%);不同种质、生理年龄的铁皮石斛多糖质量分数存在显著差异,5号种质多糖质量分数最低,一年生、二年生、三年生多糖质量分数分别为19.77%,25.63%,11.83%,10号种质最高,一年生、二年生、三年生多糖质量分数分别为33.31%,41.35%,28.39%。 结论 :全国人工栽培骨干基地生产的铁皮石斛,其多糖质量分数普遍高于野生药材,种质与生长年限显著影响多糖质量分数;铁皮石斛品种选育与采收年限的控制可提高多糖质量分数,浙江产区铁皮石斛最佳采收时间为两年生冬季至三年生开花前。
中文关键词:铁皮石斛  种质  形态特征  生理年龄  总多糖  苯酚-硫酸法
 
Quantitive variation of polysaccharides content in cultivatedDendrobium candidum
Abstract:Objective : To reveal the variation content of polysaccharides in cultivated Dendrobium candidum and the relationship between germplasms, harvesting and polysaccharides content for the breeding of quality of D. candidium. Method : The morphological characteristics were recorded when 33 samples were collected. The content of polysaccharides was determined by phenol-sulphuric acid method. Result : The average content of polysaccharides in 2-year-old samples was 34.47% (25.63%-41.65%). The polysaccharides content of samples were significantly different among germplasms and physiological ages. Conclusion : The polysaccharides content of cultivated D. candidum is higher than that of wild materials. Germplasms and physiological age impact on the polysaccharides content significantly. D. candidum breeding and the control of harvesting can increase polysaccharides content.
keywords:Dendrobium candidum  germplasm  biological characteristics  phenol-sulphuric acid method
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