中药材DNA条形码分子鉴定指导原则
投稿时间:2013-01-05    责任编辑:  点此下载全文
引用本文:陈士林,姚辉,韩建萍,辛天怡,庞晓慧,石林春,罗焜,宋经元,侯典云,石上梅,钱忠直.中药材DNA条形码分子鉴定指导原则[J].中国中药杂志,2013,38(2):141.
DOI:10.4268/cjcmm20130201
摘要点击次数: 2207
全文下载次数: 149
                                
作者中文名作者英文名单位中文名单位英文名E-Mail
陈士林 CHEN Shi-lin 中国医学科学院 北京协和医学院 药用植物研究所 濒危药材繁育国家工程实验室, 北京 100193
中国中医科学院 中药研究所, 北京 100700
The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
slchen@implad.ac.cn 
姚辉 YAO Hui 中国医学科学院 北京协和医学院 药用植物研究所 濒危药材繁育国家工程实验室, 北京 100193 The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
韩建萍 HAN Jian-ping 中国医学科学院 北京协和医学院 药用植物研究所 濒危药材繁育国家工程实验室, 北京 100193 The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
辛天怡 XIN Tian-yi 中国医学科学院 北京协和医学院 药用植物研究所 濒危药材繁育国家工程实验室, 北京 100193 The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
庞晓慧 PANG Xiao-hui 中国医学科学院 北京协和医学院 药用植物研究所 濒危药材繁育国家工程实验室, 北京 100193 The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
石林春 SHI Lin-chun 中国医学科学院 北京协和医学院 药用植物研究所 濒危药材繁育国家工程实验室, 北京 100193 The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
罗焜 LUO Kun 中国医学科学院 北京协和医学院 药用植物研究所 濒危药材繁育国家工程实验室, 北京 100193 The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
宋经元 SONG Jing-yuan 中国医学科学院 北京协和医学院 药用植物研究所 濒危药材繁育国家工程实验室, 北京 100193 The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
侯典云 HOU Dian-yun 中国医学科学院 北京协和医学院 药用植物研究所 濒危药材繁育国家工程实验室, 北京 100193 The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
石上梅 SHI Shang-mei 国家药典委员会, 北京 100061 Chinese Pharmacopoeia Commission, Beijing 100061, China  
钱忠直 QIAN Zhong-zhi 国家药典委员会, 北京 100061 Chinese Pharmacopoeia Commission, Beijing 100061, China  
基金项目:国家自然科学基金项目(81130069); 国家高技术研究发展计划(863)项目(2012AA021602); 教育部长江学者和创新团队发展计划项目(IRT1150)
中文摘要:随着中药材DNA条形码分子鉴定方法研究的深入与普及,国家药典委员会讨论通过在《中国药典》增补本中列入中药材DNA条形码分子鉴定指导原则,该指导原则通过对大样本量中药材进行DNA条形码分子鉴定研究,建立以ITS2为核心,psbA-trnH为辅的植物类药材DNA条形码鉴定体系和以COI为主、ITS2为辅的动物类药材DNA条形码鉴定体系。该文介绍了中药材DNA条形码分子鉴定指导原则及其起草说明,并对该原则在中药材鉴定方面的应用前景进行展望。
中文关键词:中国药典  中药材  DNA条形码  分子鉴定  指导原则
 
Principles for molecular identification of traditional Chinese materia medica using DNA barcoding
Abstract:Since the research of molecular identification of Chinese Materia Medica (CMM) using DNA barcode is rapidly developing and popularizing, the principle of this method is approved to be listed in the Supplement of the Pharmacopoeia of the People's Republic of China. Based on the study on comprehensive samples, the DNA barcoding systems have been established to identify CMM, i.e. ITS2 as a core barcode and psbA-trnH as a complementary locus for identification of planta medica, and COI as a core barcode and ITS2 as a complementary locus for identification of animal medica. This article introduced the principle of molecular identification of CMM using DNA barcoding and its drafting instructions. Furthermore, its application perspective was discussed.
keywords:Chinese pharmacopoeia  traditional Chinese materia medica  DNA barcoding  molecular identification  principle
查看全文   下载PDF阅读器
相关阅读
评论
zuo-jiat you-jiat