睡眠剥夺诱导的大鼠血瘀证对血小板活化影响的研究
投稿时间:2018-04-26  修订日期:2018-05-16  责任编辑:  点此下载全文
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作者中文名作者英文名单位中文名单位英文名E-Mail
史雅红 shi yahong 中国中医科学院西苑医院 Xiyuan Hospital of China Academy of Chinese Medical Sciences syh11845@126.com 
任建勋 Ren Jianxun 中国中医科学院西苑医院 Xiyuan Hospital of China Academy of Chinese Medical Sciences  
刘建勋* Liu Jianxun 中国中医科学院西苑医院 Xiyuan Hospital of China Academy of Chinese Medical Sciences liujx0324@sina.com 
黄婷婷 Huang Tingting 中国中医科学院西苑医院 Xiyuan Hospital of China Academy of Chinese Medical Sciences  
郝婷婷 Hao Tingting 中国中医科学院西苑医院 Xiyuan Hospital of China Academy of Chinese Medical Sciences  
王敏 Wang Min 中国中医科学院西苑医院 Xiyuan Hospital of China Academy of Chinese Medical Sciences  
李军梅 Li Junmei 中国中医科学院西苑医院 Xiyuan Hospital of China Academy of Chinese Medical Sciences  
林成仁 Lin Chengren 中国中医科学院西苑医院 Xiyuan Hospital of China Academy of Chinese Medical Sciences  
任钧国 Ren Junguo 中国中医科学院西苑医院 Xiyuan Hospital of China Academy of Chinese Medical Sciences  
基金项目:国 家 重 点 基 础 研 究 发 展 计 划 ( 973 计 划) 项 目( 2015CB554405)
中文摘要:运用睡眠剥夺法诱导大鼠血瘀证模型,检测中医证候宏观指标体重、舌象及血液流变学确定中医血瘀证候,运用酶联免疫法检测血小板α颗粒、致密颗粒释放及活化相关指标,为证候研究的血小板功能评价及相关药物影响提供实验基础。结果显示,模型组大鼠体重较正常组明显降低(P<0.01);舌象呈现紫暗的血瘀证特点,色彩分析舌面R、G、B值较正常组明显降低(P<0.01);血液流变学高切、中切、低切全血粘度均高于正常组(P<0.01),血浆粘度未有明显变化;血小板α颗粒(GMP-140、β-TG、PF4)及致密颗粒(ADP、5-HT)释放明显(P<0.01)。血浆TXB2和6-keto-PGF1α都较正常组明显升高(P<0.01),且两者比值也显著高于正常组(P<0.01)。PAF模型组较正常组明显上升(P<0.01)。表明睡眠剥夺诱导的大鼠血瘀证血小板功能存在明显改变,血小板活化增强,颗粒释放增多,并可能存在炎症及内皮细胞功能异常。
中文关键词:睡眠剥夺  血瘀  证候  血小板  颗粒释放
 
Effects of Sleep deprivation induced Blood stasis Syndrome on Platelet Activation in Rats
Abstract:Blood stasis syndrome is the pre-state of thrombotic disease. We use the model of blood stasis syndrome in rats was induced by sleep deprivation to study on effects of blood stasis syndrome on platelet activation. The weight, the color of tongue and hemorheology for the blood stasis syndrome of Chinese medicine were measured after modeling. The release of platelet granules and platelet activation factors in plasma were detected by ELISA kit related indicators provide experimental basis for platelet function evaluation and related drug effects in syndrome research. The results showed that the weight of the model group rats was significantly lower than that of the normal group (P<0.01).The tongue showed a dark purple blood stasis pattern, and the R, G, and B values of the tongue surface in model group were significantly lower than those of the normal group (P<0.01). The hemorheological parameters including high shear, midddle shear and low shear viscosity in whole blood were significantly higher than those in the normal group (P <0.01). But plasma viscosity did not change significantly; The release levels of platelet α particles (GMP-140, β-TG, PF4) and dense particles (ADP, 5-HT) were significantly higher than those in the normal group (P<0.01). The levels of TXB2 and 6-keto-PGF1a in plasma were significantly higher than those in the normal group (P<0.01).The ratios of TXB2 and 6-keto-PGF1α were also significantly higher than those in the normal group (P<0.01). The levels of PAF in plasma in model group were significantly higher than those in the normal group (P<0.01). It was concluded that platelet functions could be changed induced by sleep deprivation in rats with blood stasis syndrome, and there might be inflammation and endothelial cell dysfunction.
keywords:sleep deprivation  blood stasis  syndrome  platelet  granule release
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