基于指纹图谱和多组分含量测定的蒲公英药材质量控制研究
投稿时间:2018-04-25  修订日期:2018-05-25  责任编辑:  点此下载全文
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作者中文名作者英文名单位中文名单位英文名E-Mail
刘爱朋 Liu aipeng 河北中医学院 Hebei University of Chinese Medicine liuaipeng1127@163.com 
郭利霄 Guo lixiao 河北中医学院 Hebei University of Chinese Medicine  
薛紫鲸 Xue zijing 河北中医学院 Hebei University of Chinese Medicine  
郑玉光 Zheng yuguang 河北中医学院 Hebei University of Chinese Medicine  
郭龙* Guolong 河北中医学院 Hebei University of Chinese Medicine guo_long11@163.com 
基金项目:孙宝惠全国名老中医药专家传承工作室(7002016008005);河北省中医药管理局科研计划项目(2018108);河北省自然科学基金(H2018423032);河北省现代农业产业技术体系中药材产业创新团队
中文摘要:蒲公英为菊科植物蒲公英Taraxacum mongolicum Hand.-Mazz.、碱地蒲公英 Tarxacum borealisinense Kitam.或同属数种植物的干燥全草。根据生长栽培模式不同,蒲公英药材可分为野生品和栽培品两种。本文采用高效液相色谱法对11批蒲公英野生品和9批蒲公英栽培品的化学成分进行了分析,建立了蒲公英药材的化学指纹图谱,共标定9个共有峰。各批蒲公英样品相似度均在0.960之上,所建立的蒲公英化学指纹图谱专属性良好,可用于蒲公英药材的质量评价。在此基础上,进一步对蒲公英野生品和栽培品中的单咖啡酰酒石酸、绿原酸、咖啡酸、菊苣酸、异绿原酸A、木犀草素6种化学成分进行了含量测定,并结合主成分分析和正交偏最小二乘判别分析等化学计量学方法对蒲公英野生品和栽培品药材进行区分与比较。结果表明蒲公英野生品和栽培品化学成分间存在一定的差异,异绿原酸A和木犀草素是两类蒲公英野生品和栽培品的差异化合物,可作为鉴别和区分二者的质量控制指标。通过以上研究,为蒲公英药材的质量控制和蒲公英野生品和栽培品的区分提供了分析方法和数据支撑。
中文关键词:蒲公英  指纹图谱  含量测定  化学计量学  质量控制
 
Quality evaluation of Taraxaci Herba based on fingerprint analysis and quantitative analysis of multi-components
Abstract:Taraxaci Herba, the dried Herba of Taraxacum mongolicum Hand.-Mazz., Tarxacum borealisinense Kitam. and several species from the Taraxacum genus. Taraxaci Herba has been widely used in traditional Chinese and folk medicines. According to the different growth and cultivation pattern, Taraxaci Herba could be divided into two species, wild Taraxaci Herba and cultivated Taraxaci Herba. In the present study, an accurate and reliable fingerint approach was developed using high performance liquid chromatography for quality control of Taraxaci Herba. A total of 9 common peaks were marked, and the similarity of all the Taraxaci Herba samples was above 0.960. The established fingerprint method could be used for quality control of Taraxaci Herba. Furthermore, a high performance liquid chromatography method was established for simultaneous determination of six bioactive compounds, including monocaffeoyl tartaric acid, chlorogenic acid, caffeic acid, cichoric acid, 4,5-dicaffeoylquinic acid and luteolin in wild Taraxaci Herba and cultivated Taraxaci Herba. Moreover,chemometrics analysis such as principal component analysis and orthogonal partial least squares discriminant analysis were performed to compare and discriminate the wild Taraxaci Herba and cultivated Taraxaci Herba based on the quantitative data. The chemometrics results indicated that 4,5-dicaffeoylquinic acid and luteolin were significant to effectively discriminate the wild Taraxaci Herba and cultivated Taraxaci Herba samples, and these two compounds could be recognized as chemical markers for quality evaluation of wild Taraxaci Herba and cultivated Taraxaci Herba. The fingerprint analysis and quantitative analysis of multi-components could be a well-acceptable strategy for evaluation the quality of Taraxaci Herba.
keywords:Taraxaci Herba  fingerprint analysis  quantitative analysis  Chemometrics  quality evaluation
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