银杏二萜内酯K抗血小板聚集及神经保护作用研究
投稿时间:2017-12-04    责任编辑:  点此下载全文
引用本文:陶晓倩,曹泽彧,曹亮,萧伟.银杏二萜内酯K抗血小板聚集及神经保护作用研究[J].中国中药杂志,2017,42(24):4727.
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作者中文名作者英文名单位中文名单位英文名E-Mail
陶晓倩 TAO Xiao-qian 江苏康缘药业股份有限公司 中药制药过程新技术国家重点实验室, 江苏 连云港 222001 State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Parmaceutical Co., Ltd., Lianyungang 222001, China  
曹泽彧 CAO Ze-yu 江苏康缘药业股份有限公司 中药制药过程新技术国家重点实验室, 江苏 连云港 222001 State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Parmaceutical Co., Ltd., Lianyungang 222001, China  
曹亮 CAO Liang 江苏康缘药业股份有限公司 中药制药过程新技术国家重点实验室, 江苏 连云港 222001 State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Parmaceutical Co., Ltd., Lianyungang 222001, China  
萧伟 XIAO Wei 江苏康缘药业股份有限公司 中药制药过程新技术国家重点实验室, 江苏 连云港 222001 State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Kanion Parmaceutical Co., Ltd., Lianyungang 222001, China kanionlunwen@163.com 
基金项目:国家"重大新药创制"科技重大专项(2013ZX09402203);江苏省科技成果转化项目(BA2010109)
中文摘要:观察不同浓度的银杏二萜内酯K(GK)对血小板活化因子(PAF)诱导血小板聚集的拮抗作用及对缺血再灌注损伤细胞和动物模型的神经保护作用。制备GK家兔含药血清,用血小板聚集功能测定法观察GK含药血清对PAF诱导的家兔血小板聚集的影响。建立缺糖缺氧复氧(OGD/R)细胞模型,采用Hoechst/PI双染考察不同浓度的GK对OGD/R损伤的SH-SY5Y细胞凋亡的影响;建立大鼠局灶性脑缺血再灌注模型(I/R),检测GK对神经行为评分及脑梗死体积的影响。GK能剂量依赖性的抑制PAF诱导的血小板聚集,逆转OGD/R损伤造成的细胞凋亡并改善大鼠脑缺血再灌注损伤后的神经行为评分及脑组织梗死体积。GK能抑制PAF诱导的血小板聚集、改善脑缺血再灌注神经损伤。
中文关键词:银杏二萜内酯K  血小板活化因子  细胞凋亡  缺血再灌注
 
Effects of ginkgolide K on platelet aggregation activity and neuroprotection
Abstract:To investigate the antagonism effects of different concentrations of ginkgolide K(GK) on platelet activating factor (PAF)-induced platelet aggregation and neuroprotective effect on cells and animal models of ischemia-reperfusion injury. GK-containing serum in rabbit was prepared, and the effects of GK-containing serum on PAF-induced platelet aggregation was observed by platelet aggregation assay. The effect of different concentrations of GK on apoptosis of SH-SY5Y cells injured by oxygen-glucose deprivation/reoxygenation (OGD/R) was investigated by Hoechst 33342/PI double staining in OGD/R cell model. The focal cerebral ischemia-reperfusion model (I/R)was established in rats to detect the effects of GK on neurobehavioral scores and cerebral infarction volume. GK could inhibit PAF-induced platelet aggregation, reverse the apoptosis induced by OGD/R injury and improve the neurobehavioral score and cerebral infarction volume after cerebral ischemia-reperfusion injury in rats in a dose-dependent manner. GK can inhibit PAF-induced platelet aggregation and improve nerve injury after cerebral ischemia-reperfusion.
keywords:ginkgolide K  platelet activating factor  apoptosis  ischemia reperfusion
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